Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly
Identifieur interne : 000067 ( an2020/Analysis ); précédent : 000066; suivant : 000068Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly
Auteurs : Stijn De Munter [Belgique] ; Alexander Van Parys [Belgique] ; Layla Bral [Belgique] ; Joline Ingels [Belgique] ; Glenn Goetgeluk [Belgique] ; Sarah Bonte [Belgique] ; Melissa Pille [Belgique] ; Lore Billiet [Belgique] ; Karin Weening [Belgique] ; Annick Verhee [Belgique] ; Jose Van Der Heyden [Belgique] ; Tom Taghon [Belgique] ; Georges Leclercq [Belgique] ; Tessa Kerre [Belgique] ; Jan Tavernier [Belgique] ; Bart Vandekerckhove [Belgique]Source :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2020.
Abstract
Recent approval of chimeric antigen receptor (CAR) T cell therapy by the European Medicines Agency (EMA)/Federal and Drug Administration (FDA) and the remarkable results of CAR T clinical trials illustrate the curative potential of this therapy. While CARs against a multitude of different antigens are being developed and tested (pre)clinically, there is still a need for optimization. The use of single-chain variable fragments (scFvs) as targeting moieties hampers the quick generation of functional CARs and could potentially limit the efficacy. Instead, nanobodies may largely circumvent these difficulties. We used an available nanobody library generated after immunization of llamas against Cluster of Differentiation (CD) 20 through DNA vaccination or against the ectodomain of CD33 using soluble protein. The nanobody specific sequences were amplified by PCR and cloned by Gibson Assembly into a retroviral vector containing two different second-generation CAR constructs. After transduction in T cells, we observed high cell membrane nanoCAR expression in all cases. Following stimulation of nanoCAR-expressing T cells with antigen-positive cell lines, robust T cell activation, cytokine production and tumor cell lysis both in vitro and in vivo was observed. The use of nanobody technology in combination with PCR and Gibson Assembly allows for the rapid and effective generation of compact CARs.
Url:
DOI: 10.3390/ijms21030883
PubMed: 32019116
PubMed Central: 7037261
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000624
- to stream Pmc, to step Curation: 000624
- to stream Pmc, to step Checkpoint: 000062
- to stream Ncbi, to step Merge: 000E83
- to stream Ncbi, to step Curation: 000E83
- to stream Ncbi, to step Checkpoint: 000E83
- to stream Main, to step Merge: 000067
- to stream Main, to step Curation: 000067
- to stream Main, to step Exploration: 000067
- to stream an2020, to step Extraction: 000067
Links to Exploration step
PMC:7037261Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly</title><author><name sortKey="De Munter, Stijn" sort="De Munter, Stijn" uniqKey="De Munter S" first="Stijn" last="De Munter">Stijn De Munter</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Parys, Alexander" sort="Van Parys, Alexander" uniqKey="Van Parys A" first="Alexander" last="Van Parys">Alexander Van Parys</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Bral, Layla" sort="Bral, Layla" uniqKey="Bral L" first="Layla" last="Bral">Layla Bral</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Ingels, Joline" sort="Ingels, Joline" uniqKey="Ingels J" first="Joline" last="Ingels">Joline Ingels</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Goetgeluk, Glenn" sort="Goetgeluk, Glenn" uniqKey="Goetgeluk G" first="Glenn" last="Goetgeluk">Glenn Goetgeluk</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Bonte, Sarah" sort="Bonte, Sarah" uniqKey="Bonte S" first="Sarah" last="Bonte">Sarah Bonte</name><affiliation wicri:level="4"><nlm:aff id="af3-ijms-21-00883">Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Pille, Melissa" sort="Pille, Melissa" uniqKey="Pille M" first="Melissa" last="Pille">Melissa Pille</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Billiet, Lore" sort="Billiet, Lore" uniqKey="Billiet L" first="Lore" last="Billiet">Lore Billiet</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Weening, Karin" sort="Weening, Karin" uniqKey="Weening K" first="Karin" last="Weening">Karin Weening</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Verhee, Annick" sort="Verhee, Annick" uniqKey="Verhee A" first="Annick" last="Verhee">Annick Verhee</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Der Heyden, Jose" sort="Van Der Heyden, Jose" uniqKey="Van Der Heyden J" first="Jose" last="Van Der Heyden">Jose Van Der Heyden</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Taghon, Tom" sort="Taghon, Tom" uniqKey="Taghon T" first="Tom" last="Taghon">Tom Taghon</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Leclercq, Georges" sort="Leclercq, Georges" uniqKey="Leclercq G" first="Georges" last="Leclercq">Georges Leclercq</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Kerre, Tessa" sort="Kerre, Tessa" uniqKey="Kerre T" first="Tessa" last="Kerre">Tessa Kerre</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="af3-ijms-21-00883">Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Tavernier, Jan" sort="Tavernier, Jan" uniqKey="Tavernier J" first="Jan" last="Tavernier">Jan Tavernier</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Vandekerckhove, Bart" sort="Vandekerckhove, Bart" uniqKey="Vandekerckhove B" first="Bart" last="Vandekerckhove">Bart Vandekerckhove</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32019116</idno><idno type="pmc">7037261</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037261</idno><idno type="RBID">PMC:7037261</idno><idno type="doi">10.3390/ijms21030883</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000624</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000624</idno><idno type="wicri:Area/Pmc/Curation">000624</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000624</idno><idno type="wicri:Area/Pmc/Checkpoint">000062</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000062</idno><idno type="wicri:Area/Ncbi/Merge">000E83</idno><idno type="wicri:Area/Ncbi/Curation">000E83</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E83</idno><idno type="wicri:Area/Main/Merge">000067</idno><idno type="wicri:Area/Main/Curation">000067</idno><idno type="wicri:Area/Main/Exploration">000067</idno><idno type="wicri:Area/an2020/Extraction">000067</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly</title><author><name sortKey="De Munter, Stijn" sort="De Munter, Stijn" uniqKey="De Munter S" first="Stijn" last="De Munter">Stijn De Munter</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Parys, Alexander" sort="Van Parys, Alexander" uniqKey="Van Parys A" first="Alexander" last="Van Parys">Alexander Van Parys</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Bral, Layla" sort="Bral, Layla" uniqKey="Bral L" first="Layla" last="Bral">Layla Bral</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Ingels, Joline" sort="Ingels, Joline" uniqKey="Ingels J" first="Joline" last="Ingels">Joline Ingels</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Goetgeluk, Glenn" sort="Goetgeluk, Glenn" uniqKey="Goetgeluk G" first="Glenn" last="Goetgeluk">Glenn Goetgeluk</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Bonte, Sarah" sort="Bonte, Sarah" uniqKey="Bonte S" first="Sarah" last="Bonte">Sarah Bonte</name><affiliation wicri:level="4"><nlm:aff id="af3-ijms-21-00883">Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Pille, Melissa" sort="Pille, Melissa" uniqKey="Pille M" first="Melissa" last="Pille">Melissa Pille</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Billiet, Lore" sort="Billiet, Lore" uniqKey="Billiet L" first="Lore" last="Billiet">Lore Billiet</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Weening, Karin" sort="Weening, Karin" uniqKey="Weening K" first="Karin" last="Weening">Karin Weening</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Verhee, Annick" sort="Verhee, Annick" uniqKey="Verhee A" first="Annick" last="Verhee">Annick Verhee</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Der Heyden, Jose" sort="Van Der Heyden, Jose" uniqKey="Van Der Heyden J" first="Jose" last="Van Der Heyden">Jose Van Der Heyden</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Taghon, Tom" sort="Taghon, Tom" uniqKey="Taghon T" first="Tom" last="Taghon">Tom Taghon</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Leclercq, Georges" sort="Leclercq, Georges" uniqKey="Leclercq G" first="Georges" last="Leclercq">Georges Leclercq</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Kerre, Tessa" sort="Kerre, Tessa" uniqKey="Kerre T" first="Tessa" last="Kerre">Tessa Kerre</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="af3-ijms-21-00883">Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Tavernier, Jan" sort="Tavernier, Jan" uniqKey="Tavernier J" first="Jan" last="Tavernier">Jan Tavernier</name><affiliation wicri:level="4"><nlm:aff id="af2-ijms-21-00883">Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Cytokine Receptor Laboratory, Flanders Institute of Biotechnology, VIB-UGent Center for Medical Biotechnology, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Vandekerckhove, Bart" sort="Vandekerckhove, Bart" uniqKey="Vandekerckhove B" first="Bart" last="Vandekerckhove">Bart Vandekerckhove</name><affiliation wicri:level="4"><nlm:aff id="af1-ijms-21-00883">Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Diagnostic Sciences, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Recent approval of chimeric antigen receptor (CAR) T cell therapy by the European Medicines Agency (EMA)/Federal and Drug Administration (FDA) and the remarkable results of CAR T clinical trials illustrate the curative potential of this therapy. While CARs against a multitude of different antigens are being developed and tested (pre)clinically, there is still a need for optimization. The use of single-chain variable fragments (scFvs) as targeting moieties hampers the quick generation of functional CARs and could potentially limit the efficacy. Instead, nanobodies may largely circumvent these difficulties. We used an available nanobody library generated after immunization of llamas against Cluster of Differentiation (CD) 20 through DNA vaccination or against the ectodomain of CD33 using soluble protein. The nanobody specific sequences were amplified by PCR and cloned by Gibson Assembly into a retroviral vector containing two different second-generation CAR constructs. After transduction in T cells, we observed high cell membrane nanoCAR expression in all cases. Following stimulation of nanoCAR-expressing T cells with antigen-positive cell lines, robust T cell activation, cytokine production and tumor cell lysis both in vitro and in vivo was observed. The use of nanobody technology in combination with PCR and Gibson Assembly allows for the rapid and effective generation of compact CARs. </p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="June, C H" uniqKey="June C">C.H. June</name></author><author><name sortKey="Sadelain, M" uniqKey="Sadelain M">M. Sadelain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maude, S L" uniqKey="Maude S">S.L. Maude</name></author><author><name sortKey="Frey, N" uniqKey="Frey N">N. Frey</name></author><author><name sortKey="Shaw, P A" uniqKey="Shaw P">P.A. Shaw</name></author><author><name sortKey="Aplenc, R" uniqKey="Aplenc R">R. Aplenc</name></author><author><name sortKey="Barrett, D M" uniqKey="Barrett D">D.M. Barrett</name></author><author><name sortKey="Bunin, N J" uniqKey="Bunin N">N.J. Bunin</name></author><author><name sortKey="Chew, A" uniqKey="Chew A">A. Chew</name></author><author><name sortKey="Gonzalez, V E" uniqKey="Gonzalez V">V.E. Gonzalez</name></author><author><name sortKey="Zheng, Z" uniqKey="Zheng Z">Z. Zheng</name></author><author><name sortKey="Lacey, S F" uniqKey="Lacey S">S.F. Lacey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, D W" uniqKey="Lee D">D.W. Lee</name></author><author><name sortKey="Kochenderfer, J N" uniqKey="Kochenderfer J">J.N. Kochenderfer</name></author><author><name sortKey="Stetler Stevenson, M" uniqKey="Stetler Stevenson M">M. Stetler-Stevenson</name></author><author><name sortKey="Cui, Y K" uniqKey="Cui Y">Y.K. Cui</name></author><author><name sortKey="Delbrook, C" uniqKey="Delbrook C">C. Delbrook</name></author><author><name sortKey="Feldman, S A" uniqKey="Feldman S">S.A. Feldman</name></author><author><name sortKey="Fry, T J" uniqKey="Fry T">T.J. Fry</name></author><author><name sortKey="Orentas, R" uniqKey="Orentas R">R. Orentas</name></author><author><name sortKey="Sabatino, M" uniqKey="Sabatino M">M. Sabatino</name></author><author><name sortKey="Shah, N N" uniqKey="Shah N">N.N. Shah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turtle, C J" uniqKey="Turtle C">C.J. Turtle</name></author><author><name sortKey="Hanafi, L A" uniqKey="Hanafi L">L.-A. Hanafi</name></author><author><name sortKey="Berger, C" uniqKey="Berger C">C. Berger</name></author><author><name sortKey="Gooley, T A" uniqKey="Gooley T">T.A. Gooley</name></author><author><name sortKey="Cherian, S" uniqKey="Cherian S">S. Cherian</name></author><author><name sortKey="Hudecek, M" uniqKey="Hudecek M">M. Hudecek</name></author><author><name sortKey="Sommermeyer, D" uniqKey="Sommermeyer D">D. Sommermeyer</name></author><author><name sortKey="Melville, K" uniqKey="Melville K">K. Melville</name></author><author><name sortKey="Pender, B" uniqKey="Pender B">B. Pender</name></author><author><name sortKey="Budiarto, T M" uniqKey="Budiarto T">T.M. Budiarto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turtle, C J" uniqKey="Turtle C">C.J. Turtle</name></author><author><name sortKey="Hanafi, L A" uniqKey="Hanafi L">L.-A. Hanafi</name></author><author><name sortKey="Berger, C" uniqKey="Berger C">C. Berger</name></author><author><name sortKey="Hudecek, M" uniqKey="Hudecek M">M. Hudecek</name></author><author><name sortKey="Pender, B" uniqKey="Pender B">B. Pender</name></author><author><name sortKey="Robinson, E" uniqKey="Robinson E">E. Robinson</name></author><author><name sortKey="Hawkins, R" uniqKey="Hawkins R">R. Hawkins</name></author><author><name sortKey="Chaney, C" uniqKey="Chaney C">C. Chaney</name></author><author><name sortKey="Cherian, S" uniqKey="Cherian S">S. Cherian</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Neelapu, S S" uniqKey="Neelapu S">S.S. Neelapu</name></author><author><name sortKey="Locke, F L" uniqKey="Locke F">F.L. Locke</name></author><author><name sortKey="Bartlett, N L" uniqKey="Bartlett N">N.L. Bartlett</name></author><author><name sortKey="Lekakis, L J" uniqKey="Lekakis L">L.J. Lekakis</name></author><author><name sortKey="Miklos, D B" uniqKey="Miklos D">D.B. Miklos</name></author><author><name sortKey="Jacobson, C A" uniqKey="Jacobson C">C.A. Jacobson</name></author><author><name sortKey="Braunschweig, I" uniqKey="Braunschweig I">I. Braunschweig</name></author><author><name sortKey="Oluwole, O O" uniqKey="Oluwole O">O.O. Oluwole</name></author><author><name sortKey="Siddiqi, T" uniqKey="Siddiqi T">T. Siddiqi</name></author><author><name sortKey="Lin, Y" uniqKey="Lin Y">Y. Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maude, S L" uniqKey="Maude S">S.L. Maude</name></author><author><name sortKey="Laetsch, T W" uniqKey="Laetsch T">T.W. Laetsch</name></author><author><name sortKey="Buechner, J" uniqKey="Buechner J">J. Buechner</name></author><author><name sortKey="Rives, S" uniqKey="Rives S">S. Rives</name></author><author><name sortKey="Boyer, M" uniqKey="Boyer M">M. Boyer</name></author><author><name sortKey="Bittencourt, H" uniqKey="Bittencourt H">H. Bittencourt</name></author><author><name sortKey="Bader, P" uniqKey="Bader P">P. Bader</name></author><author><name sortKey="Verneris, M R" uniqKey="Verneris M">M.R. Verneris</name></author><author><name sortKey="Stefanski, H E" uniqKey="Stefanski H">H.E. Stefanski</name></author><author><name sortKey="Myers, G D" uniqKey="Myers G">G.D. Myers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fry, T J" uniqKey="Fry T">T.J. Fry</name></author><author><name sortKey="Shah, N N" uniqKey="Shah N">N.N. Shah</name></author><author><name sortKey="Orentas, R J" uniqKey="Orentas R">R.J. Orentas</name></author><author><name sortKey="Stetler Stevenson, M" uniqKey="Stetler Stevenson M">M. Stetler-Stevenson</name></author><author><name sortKey="Yuan, C M" uniqKey="Yuan C">C.M. Yuan</name></author><author><name sortKey="Ramakrishna, S" uniqKey="Ramakrishna S">S. Ramakrishna</name></author><author><name sortKey="Wolters, P" uniqKey="Wolters P">P. Wolters</name></author><author><name sortKey="Martin, S" uniqKey="Martin S">S. Martin</name></author><author><name sortKey="Delbrook, C" uniqKey="Delbrook C">C. Delbrook</name></author><author><name sortKey="Yates, B" uniqKey="Yates B">B. Yates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, J H" uniqKey="Park J">J.H. Park</name></author><author><name sortKey="Riviere, I" uniqKey="Riviere I">I. Rivière</name></author><author><name sortKey="Gonen, M" uniqKey="Gonen M">M. Gonen</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author><author><name sortKey="Senechal, B" uniqKey="Senechal B">B. Sénéchal</name></author><author><name sortKey="Curran, K J" uniqKey="Curran K">K.J. Curran</name></author><author><name sortKey="Sauter, C" uniqKey="Sauter C">C. Sauter</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Santomasso, B" uniqKey="Santomasso B">B. Santomasso</name></author><author><name sortKey="Mead, E" uniqKey="Mead E">E. Mead</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schuster, S J" uniqKey="Schuster S">S.J. Schuster</name></author><author><name sortKey="Bishop, M R" uniqKey="Bishop M">M.R. Bishop</name></author><author><name sortKey="Tam, C S" uniqKey="Tam C">C.S. Tam</name></author><author><name sortKey="Waller, E K" uniqKey="Waller E">E.K. Waller</name></author><author><name sortKey="Borchmann, P" uniqKey="Borchmann P">P. Borchmann</name></author><author><name sortKey="Mcguirk, J P" uniqKey="Mcguirk J">J.P. McGuirk</name></author><author><name sortKey="J Ger, U" uniqKey="J Ger U">U. Jäger</name></author><author><name sortKey="Jaglowski, S" uniqKey="Jaglowski S">S. Jaglowski</name></author><author><name sortKey="Andreadis, C" uniqKey="Andreadis C">C. Andreadis</name></author><author><name sortKey="Westin, J R" uniqKey="Westin J">J.R. Westin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garfall, A L" uniqKey="Garfall A">A.L. Garfall</name></author><author><name sortKey="Maus, M V" uniqKey="Maus M">M.V. Maus</name></author><author><name sortKey="Hwang, W T" uniqKey="Hwang W">W.-T. Hwang</name></author><author><name sortKey="Lacey, S F" uniqKey="Lacey S">S.F. Lacey</name></author><author><name sortKey="Mahnke, Y D" uniqKey="Mahnke Y">Y.D. Mahnke</name></author><author><name sortKey="Melenhorst, J J" uniqKey="Melenhorst J">J.J. Melenhorst</name></author><author><name sortKey="Zheng, Z" uniqKey="Zheng Z">Z. Zheng</name></author><author><name sortKey="Vogl, D T" uniqKey="Vogl D">D.T. Vogl</name></author><author><name sortKey="Cohen, A D" uniqKey="Cohen A">A.D. Cohen</name></author><author><name sortKey="Weiss, B M" uniqKey="Weiss B">B.M. Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Majzner, R G" uniqKey="Majzner R">R.G. Majzner</name></author><author><name sortKey="Mackall, C L" uniqKey="Mackall C">C.L. Mackall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mueller, K T" uniqKey="Mueller K">K.T. Mueller</name></author><author><name sortKey="Maude, S L" uniqKey="Maude S">S.L. Maude</name></author><author><name sortKey="Porter, D L" uniqKey="Porter D">D.L. Porter</name></author><author><name sortKey="Frey, N" uniqKey="Frey N">N. Frey</name></author><author><name sortKey="Wood, P" uniqKey="Wood P">P. Wood</name></author><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Waldron, E" uniqKey="Waldron E">E. Waldron</name></author><author><name sortKey="Chakraborty, A" uniqKey="Chakraborty A">A. Chakraborty</name></author><author><name sortKey="Awasthi, R" uniqKey="Awasthi R">R. Awasthi</name></author><author><name sortKey="Levine, B L" uniqKey="Levine B">B.L. Levine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weatherill, E E" uniqKey="Weatherill E">E.E. Weatherill</name></author><author><name sortKey="Cain, K L" uniqKey="Cain K">K.L. Cain</name></author><author><name sortKey="Heywood, S P" uniqKey="Heywood S">S.P. Heywood</name></author><author><name sortKey="Compson, J E" uniqKey="Compson J">J.E. Compson</name></author><author><name sortKey="Heads, J T" uniqKey="Heads J">J.T. Heads</name></author><author><name sortKey="Adams, R" uniqKey="Adams R">R. Adams</name></author><author><name sortKey="Humphreys, D P" uniqKey="Humphreys D">D.P. Humphreys</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nisbet, R M" uniqKey="Nisbet R">R.M. Nisbet</name></author><author><name sortKey="Van Der Jeugd, A" uniqKey="Van Der Jeugd A">A. Van der Jeugd</name></author><author><name sortKey="Leinenga, G" uniqKey="Leinenga G">G. Leinenga</name></author><author><name sortKey="Evans, H T" uniqKey="Evans H">H.T. Evans</name></author><author><name sortKey="Janowicz, P W" uniqKey="Janowicz P">P.W. Janowicz</name></author><author><name sortKey="Gotz, J" uniqKey="Gotz J">J. Götz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, K" uniqKey="Zhang K">K. Zhang</name></author><author><name sortKey="Geddie, M L" uniqKey="Geddie M">M.L. Geddie</name></author><author><name sortKey="Kohli, N" uniqKey="Kohli N">N. Kohli</name></author><author><name sortKey="Kornaga, T" uniqKey="Kornaga T">T. Kornaga</name></author><author><name sortKey="Kirpotin, D B" uniqKey="Kirpotin D">D.B. Kirpotin</name></author><author><name sortKey="Jiao, Y" uniqKey="Jiao Y">Y. Jiao</name></author><author><name sortKey="Rennard, R" uniqKey="Rennard R">R. Rennard</name></author><author><name sortKey="Drummond, D C" uniqKey="Drummond D">D.C. Drummond</name></author><author><name sortKey="Nielsen, U B" uniqKey="Nielsen U">U.B. Nielsen</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kershaw, M H" uniqKey="Kershaw M">M.H. Kershaw</name></author><author><name sortKey="Westwood, J A" uniqKey="Westwood J">J.A. Westwood</name></author><author><name sortKey="Parker, L L" uniqKey="Parker L">L.L. Parker</name></author><author><name sortKey="Wang, G" uniqKey="Wang G">G. Wang</name></author><author><name sortKey="Eshhar, Z" uniqKey="Eshhar Z">Z. Eshhar</name></author><author><name sortKey="Mavroukakis, S A" uniqKey="Mavroukakis S">S.A. Mavroukakis</name></author><author><name sortKey="White, D E" uniqKey="White D">D.E. White</name></author><author><name sortKey="Wunderlich, J R" uniqKey="Wunderlich J">J.R. Wunderlich</name></author><author><name sortKey="Canevari, S" uniqKey="Canevari S">S. Canevari</name></author><author><name sortKey="Rogers Freezer, L" uniqKey="Rogers Freezer L">L. Rogers-Freezer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maus, M V" uniqKey="Maus M">M.V. Maus</name></author><author><name sortKey="Haas, A R" uniqKey="Haas A">A.R. Haas</name></author><author><name sortKey="Beatty, G L" uniqKey="Beatty G">G.L. Beatty</name></author><author><name sortKey="Albelda, S M" uniqKey="Albelda S">S.M. Albelda</name></author><author><name sortKey="Levine, B L" uniqKey="Levine B">B.L. Levine</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Zhao, Y" uniqKey="Zhao Y">Y. Zhao</name></author><author><name sortKey="Kalos, M" uniqKey="Kalos M">M. Kalos</name></author><author><name sortKey="June, C H" uniqKey="June C">C.H. June</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Long, A H" uniqKey="Long A">A.H. Long</name></author><author><name sortKey="Haso, W M" uniqKey="Haso W">W.M. Haso</name></author><author><name sortKey="Shern, J F" uniqKey="Shern J">J.F. Shern</name></author><author><name sortKey="Wanhainen, K M" uniqKey="Wanhainen K">K.M. Wanhainen</name></author><author><name sortKey="Murgai, M" uniqKey="Murgai M">M. Murgai</name></author><author><name sortKey="Ingaramo, M" uniqKey="Ingaramo M">M. Ingaramo</name></author><author><name sortKey="Smith, J P" uniqKey="Smith J">J.P. Smith</name></author><author><name sortKey="Walker, A J" uniqKey="Walker A">A.J. Walker</name></author><author><name sortKey="Kohler, M E" uniqKey="Kohler M">M.E. Kohler</name></author><author><name sortKey="Venkateshwara, V R" uniqKey="Venkateshwara V">V.R. Venkateshwara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nieba, L" uniqKey="Nieba L">L. Nieba</name></author><author><name sortKey="Honegger, A" uniqKey="Honegger A">A. Honegger</name></author><author><name sortKey="Krebber, C" uniqKey="Krebber C">C. Krebber</name></author><author><name sortKey="Pluckthun, A" uniqKey="Pluckthun A">A. Pluckthun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, W" uniqKey="Sun W">W. Sun</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J. Xie</name></author><author><name sortKey="Lin, H" uniqKey="Lin H">H. Lin</name></author><author><name sortKey="Mi, S" uniqKey="Mi S">S. Mi</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z. Li</name></author><author><name sortKey="Hua, F" uniqKey="Hua F">F. Hua</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z. Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sotillo, E" uniqKey="Sotillo E">E. Sotillo</name></author><author><name sortKey="Barrett, D M" uniqKey="Barrett D">D.M. Barrett</name></author><author><name sortKey="Black, K L" uniqKey="Black K">K.L. Black</name></author><author><name sortKey="Bagashev, A" uniqKey="Bagashev A">A. Bagashev</name></author><author><name sortKey="Oldridge, D" uniqKey="Oldridge D">D. Oldridge</name></author><author><name sortKey="Wu, G" uniqKey="Wu G">G. Wu</name></author><author><name sortKey="Sussman, R" uniqKey="Sussman R">R. Sussman</name></author><author><name sortKey="Lanauze, C" uniqKey="Lanauze C">C. Lanauze</name></author><author><name sortKey="Ruella, M" uniqKey="Ruella M">M. Ruella</name></author><author><name sortKey="Gazzara, M R" uniqKey="Gazzara M">M.R. Gazzara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grada, Z" uniqKey="Grada Z">Z. Grada</name></author><author><name sortKey="Hegde, M" uniqKey="Hegde M">M. Hegde</name></author><author><name sortKey="Byrd, T" uniqKey="Byrd T">T. Byrd</name></author><author><name sortKey="Shaffer, D R" uniqKey="Shaffer D">D.R. Shaffer</name></author><author><name sortKey="Ghazi, A" uniqKey="Ghazi A">A. Ghazi</name></author><author><name sortKey="Brawley, V S" uniqKey="Brawley V">V.S. Brawley</name></author><author><name sortKey="Corder, A" uniqKey="Corder A">A. Corder</name></author><author><name sortKey="Schonfeld, K" uniqKey="Schonfeld K">K. Schönfeld</name></author><author><name sortKey="Koch, J" uniqKey="Koch J">J. Koch</name></author><author><name sortKey="Dotti, G" uniqKey="Dotti G">G. Dotti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zah, E" uniqKey="Zah E">E. Zah</name></author><author><name sortKey="Lin, M Y" uniqKey="Lin M">M.-Y. Lin</name></author><author><name sortKey="Silva Benedict, A" uniqKey="Silva Benedict A">A. Silva-Benedict</name></author><author><name sortKey="Jensen, M C" uniqKey="Jensen M">M.C. Jensen</name></author><author><name sortKey="Chen, Y Y" uniqKey="Chen Y">Y.Y. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hegde, M" uniqKey="Hegde M">M. Hegde</name></author><author><name sortKey="Mukherjee, M" uniqKey="Mukherjee M">M. Mukherjee</name></author><author><name sortKey="Grada, Z" uniqKey="Grada Z">Z. Grada</name></author><author><name sortKey="Pignata, A" uniqKey="Pignata A">A. Pignata</name></author><author><name sortKey="Landi, D" uniqKey="Landi D">D. Landi</name></author><author><name sortKey="Navai, S A" uniqKey="Navai S">S.A. Navai</name></author><author><name sortKey="Wakefield, A" uniqKey="Wakefield A">A. Wakefield</name></author><author><name sortKey="Fousek, K" uniqKey="Fousek K">K. Fousek</name></author><author><name sortKey="Bielamowicz, K" uniqKey="Bielamowicz K">K. Bielamowicz</name></author><author><name sortKey="Chow, K K H" uniqKey="Chow K">K.K.H. Chow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jensen, M C" uniqKey="Jensen M">M.C. Jensen</name></author><author><name sortKey="Riddell, S R" uniqKey="Riddell S">S.R. Riddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamers Casterman, C" uniqKey="Hamers Casterman C">C. Hamers-Casterman</name></author><author><name sortKey="Atarhouch, T" uniqKey="Atarhouch T">T. Atarhouch</name></author><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author><author><name sortKey="Robinson, G" uniqKey="Robinson G">G. Robinson</name></author><author><name sortKey="Hammers, C" uniqKey="Hammers C">C. Hammers</name></author><author><name sortKey="Songa, E B" uniqKey="Songa E">E.B. Songa</name></author><author><name sortKey="Bendahman, N" uniqKey="Bendahman N">N. Bendahman</name></author><author><name sortKey="Hammers, R" uniqKey="Hammers R">R. Hammers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author><author><name sortKey="Lauwereys, M" uniqKey="Lauwereys M">M. Lauwereys</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coppieters, K" uniqKey="Coppieters K">K. Coppieters</name></author><author><name sortKey="Dreier, T" uniqKey="Dreier T">T. Dreier</name></author><author><name sortKey="Silence, K" uniqKey="Silence K">K. Silence</name></author><author><name sortKey="Haard, H D" uniqKey="Haard H">H.D. Haard</name></author><author><name sortKey="Lauwereys, M" uniqKey="Lauwereys M">M. Lauwereys</name></author><author><name sortKey="Casteels, P" uniqKey="Casteels P">P. Casteels</name></author><author><name sortKey="Beirnaert, E" uniqKey="Beirnaert E">E. Beirnaert</name></author><author><name sortKey="Jonckheere, H" uniqKey="Jonckheere H">H. Jonckheere</name></author><author><name sortKey="Wiele, C V D" uniqKey="Wiele C">C.V.D. Wiele</name></author><author><name sortKey="Staelens, L" uniqKey="Staelens L">L. Staelens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baral, T N" uniqKey="Baral T">T.N. Baral</name></author><author><name sortKey="Magez, S" uniqKey="Magez S">S. Magez</name></author><author><name sortKey="Stijlemans, B" uniqKey="Stijlemans B">B. Stijlemans</name></author><author><name sortKey="Conrath, K" uniqKey="Conrath K">K. Conrath</name></author><author><name sortKey="Vanhollebeke, B" uniqKey="Vanhollebeke B">B. Vanhollebeke</name></author><author><name sortKey="Pays, E" uniqKey="Pays E">E. Pays</name></author><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author><author><name sortKey="De Baetselier, P" uniqKey="De Baetselier P">P. De Baetselier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fleischmann, R M" uniqKey="Fleischmann R">R.M. Fleischmann</name></author><author><name sortKey="De Bruyn, S" uniqKey="De Bruyn S">S. De Bruyn</name></author><author><name sortKey="Duby, C" uniqKey="Duby C">C. Duby</name></author><author><name sortKey="Verschueren, K" uniqKey="Verschueren K">K. Verschueren</name></author><author><name sortKey="Baumeister, J" uniqKey="Baumeister J">J. Baumeister</name></author><author><name sortKey="Sargentini Maier, L" uniqKey="Sargentini Maier L">L. Sargentini-Maier</name></author><author><name sortKey="Ververken, C" uniqKey="Ververken C">C. Ververken</name></author><author><name sortKey="Holz, J B" uniqKey="Holz J">J.-B. Holz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peyvandi, F" uniqKey="Peyvandi F">F. Peyvandi</name></author><author><name sortKey="Scully, M" uniqKey="Scully M">M. Scully</name></author><author><name sortKey="Kremer Hovinga, J A" uniqKey="Kremer Hovinga J">J.A. Kremer Hovinga</name></author><author><name sortKey="Cataland, S" uniqKey="Cataland S">S. Cataland</name></author><author><name sortKey="Knobl, P" uniqKey="Knobl P">P. Knöbl</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H. Wu</name></author><author><name sortKey="Artoni, A" uniqKey="Artoni A">A. Artoni</name></author><author><name sortKey="Westwood, J P" uniqKey="Westwood J">J.-P. Westwood</name></author><author><name sortKey="Mansouri Taleghani, M" uniqKey="Mansouri Taleghani M">M. Mansouri Taleghani</name></author><author><name sortKey="Jilma, B" uniqKey="Jilma B">B. Jilma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vincke, C" uniqKey="Vincke C">C. Vincke</name></author><author><name sortKey="Loris, R" uniqKey="Loris R">R. Loris</name></author><author><name sortKey="Saerens, D" uniqKey="Saerens D">D. Saerens</name></author><author><name sortKey="Martinez Rodriguez, S" uniqKey="Martinez Rodriguez S">S. Martinez-Rodriguez</name></author><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author><author><name sortKey="Conrath, K" uniqKey="Conrath K">K. Conrath</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conrath, K E" uniqKey="Conrath K">K.E. Conrath</name></author><author><name sortKey="Lauwereys, M" uniqKey="Lauwereys M">M. Lauwereys</name></author><author><name sortKey="Wyns, L" uniqKey="Wyns L">L. Wyns</name></author><author><name sortKey="Muyldermans, S" uniqKey="Muyldermans S">S. Muyldermans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morrison, C" uniqKey="Morrison C">C. Morrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Iri Sofla, F J" uniqKey="Iri Sofla F">F.J. Iri-Sofla</name></author><author><name sortKey="Rahbarizadeh, F" uniqKey="Rahbarizadeh F">F. Rahbarizadeh</name></author><author><name sortKey="Ahmadvand, D" uniqKey="Ahmadvand D">D. Ahmadvand</name></author><author><name sortKey="Rasaee, M J" uniqKey="Rasaee M">M.J. Rasaee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sharifzadeh, Z" uniqKey="Sharifzadeh Z">Z. Sharifzadeh</name></author><author><name sortKey="Rahbarizadeh, F" uniqKey="Rahbarizadeh F">F. Rahbarizadeh</name></author><author><name sortKey="Shokrgozar, M A" uniqKey="Shokrgozar M">M.A. Shokrgozar</name></author><author><name sortKey="Ahmadvand, D" uniqKey="Ahmadvand D">D. Ahmadvand</name></author><author><name sortKey="Mahboudi, F" uniqKey="Mahboudi F">F. Mahboudi</name></author><author><name sortKey="Jamnani, F R" uniqKey="Jamnani F">F.R. Jamnani</name></author><author><name sortKey="Moghimi, S M" uniqKey="Moghimi S">S.M. Moghimi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jamnani, F R" uniqKey="Jamnani F">F.R. Jamnani</name></author><author><name sortKey="Rahbarizadeh, F" uniqKey="Rahbarizadeh F">F. Rahbarizadeh</name></author><author><name sortKey="Shokrgozar, M A" uniqKey="Shokrgozar M">M.A. Shokrgozar</name></author><author><name sortKey="Mahboudi, F" uniqKey="Mahboudi F">F. Mahboudi</name></author><author><name sortKey="Ahmadvand, D" uniqKey="Ahmadvand D">D. Ahmadvand</name></author><author><name sortKey="Sharifzadeh, Z" uniqKey="Sharifzadeh Z">Z. Sharifzadeh</name></author><author><name sortKey="Parhamifar, L" uniqKey="Parhamifar L">L. Parhamifar</name></author><author><name sortKey="Moghimi, S M" uniqKey="Moghimi S">S.M. Moghimi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, G" uniqKey="Zhang G">G. Zhang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Cui, H" uniqKey="Cui H">H. Cui</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author><author><name sortKey="Zhang, G" uniqKey="Zhang G">G. Zhang</name></author><author><name sortKey="Ma, J" uniqKey="Ma J">J. Ma</name></author><author><name sortKey="Han, H" uniqKey="Han H">H. Han</name></author><author><name sortKey="He, W" uniqKey="He W">W. He</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Zhao, Y" uniqKey="Zhao Y">Y. Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, Y J" uniqKey="Xie Y">Y.J. Xie</name></author><author><name sortKey="Dougan, M" uniqKey="Dougan M">M. Dougan</name></author><author><name sortKey="Jailkhani, N" uniqKey="Jailkhani N">N. Jailkhani</name></author><author><name sortKey="Ingram, J" uniqKey="Ingram J">J. Ingram</name></author><author><name sortKey="Fang, T" uniqKey="Fang T">T. Fang</name></author><author><name sortKey="Kummer, L" uniqKey="Kummer L">L. Kummer</name></author><author><name sortKey="Momin, N" uniqKey="Momin N">N. Momin</name></author><author><name sortKey="Pishesha, N" uniqKey="Pishesha N">N. Pishesha</name></author><author><name sortKey="Rickelt, S" uniqKey="Rickelt S">S. Rickelt</name></author><author><name sortKey="Hynes, R O" uniqKey="Hynes R">R.O. Hynes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Munter, S" uniqKey="De Munter S">S. De Munter</name></author><author><name sortKey="Ingels, J" uniqKey="Ingels J">J. Ingels</name></author><author><name sortKey="Goetgeluk, G" uniqKey="Goetgeluk G">G. Goetgeluk</name></author><author><name sortKey="Bonte, S" uniqKey="Bonte S">S. Bonte</name></author><author><name sortKey="Pille, M" uniqKey="Pille M">M. Pille</name></author><author><name sortKey="Weening, K" uniqKey="Weening K">K. Weening</name></author><author><name sortKey="Kerre, T" uniqKey="Kerre T">T. Kerre</name></author><author><name sortKey="Abken, H" uniqKey="Abken H">H. Abken</name></author><author><name sortKey="Vandekerckhove, B" uniqKey="Vandekerckhove B">B. Vandekerckhove</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, J" uniqKey="Xu J">J. Xu</name></author><author><name sortKey="Chen, L J" uniqKey="Chen L">L.-J. Chen</name></author><author><name sortKey="Yang, S S" uniqKey="Yang S">S.-S. Yang</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y. Sun</name></author><author><name sortKey="Wu, W" uniqKey="Wu W">W. Wu</name></author><author><name sortKey="Liu, Y F" uniqKey="Liu Y">Y.-F. Liu</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J. Xu</name></author><author><name sortKey="Zhuang, Y" uniqKey="Zhuang Y">Y. Zhuang</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name></author><author><name sortKey="Weng, X Q" uniqKey="Weng X">X.-Q. Weng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kenderian, S S" uniqKey="Kenderian S">S.S. Kenderian</name></author><author><name sortKey="Ruella, M" uniqKey="Ruella M">M. Ruella</name></author><author><name sortKey="Shestova, O" uniqKey="Shestova O">O. Shestova</name></author><author><name sortKey="Klichinsky, M" uniqKey="Klichinsky M">M. Klichinsky</name></author><author><name sortKey="Aikawa, V" uniqKey="Aikawa V">V. Aikawa</name></author><author><name sortKey="Morrissette, J J D" uniqKey="Morrissette J">J.J.D. Morrissette</name></author><author><name sortKey="Scholler, J" uniqKey="Scholler J">J. Scholler</name></author><author><name sortKey="Song, D" uniqKey="Song D">D. Song</name></author><author><name sortKey="Porter, D L" uniqKey="Porter D">D.L. Porter</name></author><author><name sortKey="Carroll, M" uniqKey="Carroll M">M. Carroll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, X" uniqKey="Wu X">X. Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mitchell, R S" uniqKey="Mitchell R">R.S. Mitchell</name></author><author><name sortKey="Beitzel, B F" uniqKey="Beitzel B">B.F. Beitzel</name></author><author><name sortKey="Schroder, A R W" uniqKey="Schroder A">A.R.W. Schroder</name></author><author><name sortKey="Shinn, P" uniqKey="Shinn P">P. Shinn</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Berry, C C" uniqKey="Berry C">C.C. Berry</name></author><author><name sortKey="Ecker, J R" uniqKey="Ecker J">J.R. Ecker</name></author><author><name sortKey="Bushman, F D" uniqKey="Bushman F">F.D. Bushman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Re, F" uniqKey="Re F">F. Re</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salter, A I" uniqKey="Salter A">A.I. Salter</name></author><author><name sortKey="Ivey, R G" uniqKey="Ivey R">R.G. Ivey</name></author><author><name sortKey="Kennedy, J J" uniqKey="Kennedy J">J.J. Kennedy</name></author><author><name sortKey="Voillet, V" uniqKey="Voillet V">V. Voillet</name></author><author><name sortKey="Rajan, A" uniqKey="Rajan A">A. Rajan</name></author><author><name sortKey="Alderman, E J" uniqKey="Alderman E">E.J. Alderman</name></author><author><name sortKey="Voytovich, U J" uniqKey="Voytovich U">U.J. Voytovich</name></author><author><name sortKey="Lin, C" uniqKey="Lin C">C. Lin</name></author><author><name sortKey="Sommermeyer, D" uniqKey="Sommermeyer D">D. Sommermeyer</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawalekar, O U" uniqKey="Kawalekar O">O.U. Kawalekar</name></author><author><name sortKey="O Onnor, R S" uniqKey="O Onnor R">R.S. O’Connor</name></author><author><name sortKey="Fraietta, J A" uniqKey="Fraietta J">J.A. Fraietta</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L. Guo</name></author><author><name sortKey="Mcgettigan, S E" uniqKey="Mcgettigan S">S.E. McGettigan</name></author><author><name sortKey="Posey, A D" uniqKey="Posey A">A.D. Posey</name></author><author><name sortKey="Patel, P R" uniqKey="Patel P">P.R. Patel</name></author><author><name sortKey="Guedan, S" uniqKey="Guedan S">S. Guedan</name></author><author><name sortKey="Scholler, J" uniqKey="Scholler J">J. Scholler</name></author><author><name sortKey="Keith, B" uniqKey="Keith B">B. Keith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guest, R D" uniqKey="Guest R">R.D. Guest</name></author><author><name sortKey="Hawkins, R E" uniqKey="Hawkins R">R.E. Hawkins</name></author><author><name sortKey="Kirillova, N" uniqKey="Kirillova N">N. Kirillova</name></author><author><name sortKey="Cheadle, E J" uniqKey="Cheadle E">E.J. Cheadle</name></author><author><name sortKey="Arnold, J" uniqKey="Arnold J">J. Arnold</name></author><author><name sortKey="O Neill, A" uniqKey="O Neill A">A. O??Neill</name></author><author><name sortKey="Irlam, J" uniqKey="Irlam J">J. Irlam</name></author><author><name sortKey="Chester, K A" uniqKey="Chester K">K.A. Chester</name></author><author><name sortKey="Kemshead, J T" uniqKey="Kemshead J">J.T. Kemshead</name></author><author><name sortKey="Shaw, D M" uniqKey="Shaw D">D.M. Shaw</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haso, W" uniqKey="Haso W">W. Haso</name></author><author><name sortKey="Lee, D W" uniqKey="Lee D">D.W. Lee</name></author><author><name sortKey="Shah, N N" uniqKey="Shah N">N.N. Shah</name></author><author><name sortKey="Stetler Stevenson, M" uniqKey="Stetler Stevenson M">M. Stetler-stevenson</name></author><author><name sortKey="Yuan, C M" uniqKey="Yuan C">C.M. Yuan</name></author><author><name sortKey="Pastan, I H" uniqKey="Pastan I">I.H. Pastan</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Morgan, R A" uniqKey="Morgan R">R.A. Morgan</name></author><author><name sortKey="Fitzgerald, D J" uniqKey="Fitzgerald D">D.J. Fitzgerald</name></author><author><name sortKey="Barrett, D M" uniqKey="Barrett D">D.M. Barrett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hudecek, M" uniqKey="Hudecek M">M. Hudecek</name></author><author><name sortKey="Lupo Stanghellini, M T" uniqKey="Lupo Stanghellini M">M.T. Lupo-Stanghellini</name></author><author><name sortKey="Kosasih, P L" uniqKey="Kosasih P">P.L. Kosasih</name></author><author><name sortKey="Sommermeyer, D" uniqKey="Sommermeyer D">D. Sommermeyer</name></author><author><name sortKey="Jensen, M C" uniqKey="Jensen M">M.C. Jensen</name></author><author><name sortKey="Rader, C" uniqKey="Rader C">C. Rader</name></author><author><name sortKey="Riddell, S R" uniqKey="Riddell S">S.R. Riddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hudecek, M" uniqKey="Hudecek M">M. Hudecek</name></author><author><name sortKey="Sommermeyer, D" uniqKey="Sommermeyer D">D. Sommermeyer</name></author><author><name sortKey="Kosasih, P L" uniqKey="Kosasih P">P.L. Kosasih</name></author><author><name sortKey="Silva Benedict, A" uniqKey="Silva Benedict A">A. Silva-Benedict</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L. Liu</name></author><author><name sortKey="Rader, C" uniqKey="Rader C">C. Rader</name></author><author><name sortKey="Jensen, M C" uniqKey="Jensen M">M.C. Jensen</name></author><author><name sortKey="Riddell, S R" uniqKey="Riddell S">S.R. Riddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hombach, A" uniqKey="Hombach A">A. Hombach</name></author><author><name sortKey="Hombach, A A" uniqKey="Hombach A">A.A. Hombach</name></author><author><name sortKey="Abken, H" uniqKey="Abken H">H. Abken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alm Sbak, H" uniqKey="Alm Sbak H">H. Almåsbak</name></author><author><name sortKey="Walseng, E" uniqKey="Walseng E">E. Walseng</name></author><author><name sortKey="Kristian, A" uniqKey="Kristian A">A. Kristian</name></author><author><name sortKey="Myhre, M R" uniqKey="Myhre M">M.R. Myhre</name></author><author><name sortKey="Suso, E M" uniqKey="Suso E">E.M. Suso</name></author><author><name sortKey="Munthe, L A" uniqKey="Munthe L">L.A. Munthe</name></author><author><name sortKey="Andersen, J T" uniqKey="Andersen J">J.T. Andersen</name></author><author><name sortKey="Wang, M Y" uniqKey="Wang M">M.Y. Wang</name></author><author><name sortKey="Kvalheim, G" uniqKey="Kvalheim G">G. Kvalheim</name></author><author><name sortKey="Gaudernack, G" uniqKey="Gaudernack G">G. Gaudernack</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jonnalagadda, M" uniqKey="Jonnalagadda M">M. Jonnalagadda</name></author><author><name sortKey="Mardiros, A" uniqKey="Mardiros A">A. Mardiros</name></author><author><name sortKey="Urak, R" uniqKey="Urak R">R. Urak</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author><author><name sortKey="Hoffman, L J" uniqKey="Hoffman L">L.J. Hoffman</name></author><author><name sortKey="Bernanke, A" uniqKey="Bernanke A">A. Bernanke</name></author><author><name sortKey="Chang, W C" uniqKey="Chang W">W.-C. Chang</name></author><author><name sortKey="Bretzlaff, W" uniqKey="Bretzlaff W">W. Bretzlaff</name></author><author><name sortKey="Starr, R" uniqKey="Starr R">R. Starr</name></author><author><name sortKey="Priceman, S" uniqKey="Priceman S">S. Priceman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, N" uniqKey="Watanabe N">N. Watanabe</name></author><author><name sortKey="Bajgain, P" uniqKey="Bajgain P">P. Bajgain</name></author><author><name sortKey="Sukumaran, S" uniqKey="Sukumaran S">S. Sukumaran</name></author><author><name sortKey="Ansari, S" uniqKey="Ansari S">S. Ansari</name></author><author><name sortKey="Heslop, H E" uniqKey="Heslop H">H.E. Heslop</name></author><author><name sortKey="Rooney, C M" uniqKey="Rooney C">C.M. Rooney</name></author><author><name sortKey="Brenner, M K" uniqKey="Brenner M">M.K. Brenner</name></author><author><name sortKey="Leen, A M" uniqKey="Leen A">A.M. Leen</name></author><author><name sortKey="Vera, J F" uniqKey="Vera J">J.F. Vera</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zabeau, L" uniqKey="Zabeau L">L. Zabeau</name></author><author><name sortKey="Verhee, A" uniqKey="Verhee A">A. Verhee</name></author><author><name sortKey="Catteeuw, D" uniqKey="Catteeuw D">D. Catteeuw</name></author><author><name sortKey="Faes, L" uniqKey="Faes L">L. Faes</name></author><author><name sortKey="Seeuws, S" uniqKey="Seeuws S">S. Seeuws</name></author><author><name sortKey="Decruy, T" uniqKey="Decruy T">T. Decruy</name></author><author><name sortKey="Elewaut, D" uniqKey="Elewaut D">D. Elewaut</name></author><author><name sortKey="Peelman, F" uniqKey="Peelman F">F. Peelman</name></author><author><name sortKey="Tavernier, J" uniqKey="Tavernier J">J. Tavernier</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="Belgique"><region name="Province de Flandre-Orientale"><name sortKey="De Munter, Stijn" sort="De Munter, Stijn" uniqKey="De Munter S" first="Stijn" last="De Munter">Stijn De Munter</name></region><name sortKey="Billiet, Lore" sort="Billiet, Lore" uniqKey="Billiet L" first="Lore" last="Billiet">Lore Billiet</name><name sortKey="Bonte, Sarah" sort="Bonte, Sarah" uniqKey="Bonte S" first="Sarah" last="Bonte">Sarah Bonte</name><name sortKey="Bral, Layla" sort="Bral, Layla" uniqKey="Bral L" first="Layla" last="Bral">Layla Bral</name><name sortKey="Goetgeluk, Glenn" sort="Goetgeluk, Glenn" uniqKey="Goetgeluk G" first="Glenn" last="Goetgeluk">Glenn Goetgeluk</name><name sortKey="Ingels, Joline" sort="Ingels, Joline" uniqKey="Ingels J" first="Joline" last="Ingels">Joline Ingels</name><name sortKey="Kerre, Tessa" sort="Kerre, Tessa" uniqKey="Kerre T" first="Tessa" last="Kerre">Tessa Kerre</name><name sortKey="Kerre, Tessa" sort="Kerre, Tessa" uniqKey="Kerre T" first="Tessa" last="Kerre">Tessa Kerre</name><name sortKey="Leclercq, Georges" sort="Leclercq, Georges" uniqKey="Leclercq G" first="Georges" last="Leclercq">Georges Leclercq</name><name sortKey="Pille, Melissa" sort="Pille, Melissa" uniqKey="Pille M" first="Melissa" last="Pille">Melissa Pille</name><name sortKey="Taghon, Tom" sort="Taghon, Tom" uniqKey="Taghon T" first="Tom" last="Taghon">Tom Taghon</name><name sortKey="Tavernier, Jan" sort="Tavernier, Jan" uniqKey="Tavernier J" first="Jan" last="Tavernier">Jan Tavernier</name><name sortKey="Van Der Heyden, Jose" sort="Van Der Heyden, Jose" uniqKey="Van Der Heyden J" first="Jose" last="Van Der Heyden">Jose Van Der Heyden</name><name sortKey="Van Parys, Alexander" sort="Van Parys, Alexander" uniqKey="Van Parys A" first="Alexander" last="Van Parys">Alexander Van Parys</name><name sortKey="Vandekerckhove, Bart" sort="Vandekerckhove, Bart" uniqKey="Vandekerckhove B" first="Bart" last="Vandekerckhove">Bart Vandekerckhove</name><name sortKey="Verhee, Annick" sort="Verhee, Annick" uniqKey="Verhee A" first="Annick" last="Verhee">Annick Verhee</name><name sortKey="Weening, Karin" sort="Weening, Karin" uniqKey="Weening K" first="Karin" last="Weening">Karin Weening</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/an2020/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000067 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/an2020/Analysis/biblio.hfd -nk 000067 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= an2020
|étape= Analysis
|type= RBID
|clé= PMC:7037261
|texte= Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/an2020/Analysis/RBID.i -Sk "pubmed:32019116" \
| HfdSelect -Kh $EXPLOR_AREA/Data/an2020/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |